Journal: Dose-Response

Article Title: Collaborative Study of Thresholds for Mutagens: Hormetic Responses in Cell Proliferation Tests Using Human and Murine Lymphoid Cells

doi: 10.1177/15593258211028473

Figure Lengend Snippet: Doubling time of TK6 and L5178Y cells and hormetic responses observed in L5178Y cells treated with MMC. A, Cells (50,000 cells/0.5 mL/well) were inoculated in 6 wells (B1 to B6) of a 24-well plate at 0 h and in another 6 wells (C1 to C6) 12 h after the first inoculation. An aliquot (50 μL) was sampled 18, 24, 42, and 48 h from wells B1 to B6 and 6, 12 , 30, and 36 h from wells C1 to C6. The blue line denotes the growth of L5178Y and the red line denotes TK6. B, L5178Y cells (100,000/well/500 μL) were inoculated in a 24-well plate, treated with MMC (highest dose level of 40 ng/mL) 12 h after inoculation, and 50 µL were sampled from the same well at 6 and 9 h after treatment. C, L5178Y cells (200,000/well/500 μL) were inoculated in a 24-well plate, treated with MMC (highest dose level of 40 ng/mL) 12 h after inoculation, and 50 µL were sampled from the same well at 6,9 and 12 h after treatment. Three wells were analyzed at each time point. Vertical bars denote standard deviations from the mean. Statistically significant difference: * ( P < 0.05); **( P < 0.01).

Article Snippet: Human lymphoblastoid cells (TK6) and mouse lymphoma cells (L5178Y) were obtained from the JCRB Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.

Techniques:

Journal: Dose-Response

Article Title: Collaborative Study of Thresholds for Mutagens: Hormetic Responses in Cell Proliferation Tests Using Human and Murine Lymphoid Cells

doi: 10.1177/15593258211028473

Figure Lengend Snippet: Hormetic responses observed in L5178Y cells treated with MMC (A and B) and in cellular activity detected using a coloration reagent (C). A, Cells (100,000/well/250 μL) were inoculated in a 48-well plate, treated with MMC (highest dose level of 400 ng/mL) 14 h after inoculation, and 50 µL aliquots were sampled at 6, 8, and 10 h after treatment. B, Cells (100,000/well/250 μL) were inoculated in three 48-well plates and treated with MMC 13 h after inoculation. The 3 plates were used for sampling at 3 and 6 h, 4 and 7 h, and 5 and 8 h. C, L5178Y cells (40,000/100 μL/well) were inoculated in three 96-well plates and treated with MMC (highest dose level of 400 ng/mL) 14 h after treatment. An aliquot (50 μL) was sampled and placed into the wells of another plate and a coloration reagent, CCK-8 (5μL), was added to each well. After a coloration period of 1 h, OD 450 was measured with a scanner (see previous report for detailed method). The value at each time point was the mean of 3 wells. Vertical bars denote standard deviations from the mean. Statistically significant difference: * ( P < 0.05).

Article Snippet: Human lymphoblastoid cells (TK6) and mouse lymphoma cells (L5178Y) were obtained from the JCRB Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.

Techniques: Activity Assay, Sampling, CCK-8 Assay

Journal: Dose-Response

Article Title: Collaborative Study of Thresholds for Mutagens: Hormetic Responses in Cell Proliferation Tests Using Human and Murine Lymphoid Cells

doi: 10.1177/15593258211028473

Figure Lengend Snippet: Hormetic responses observed in L5178Y cells treated with EMS. A, Cells (100,000/well/250 μL) were inoculated in a 48-well plate, treated with EMS (highest dose level of 200 μg/mL) 13 h after inoculation, and an aliquot (50 μL) was sampled at 6, 8, and 10 h after treatment. B, Cells (200,000/well/500 μL) were inoculated in a 24-well plate, treated with EMS (highest dose level of 800 μg/mL) 14 h after inoculation, and an aliquot (50 μL) was sampled at 5, 7, and 9 h after treatment. C, Cells (200,000/well/500 μL) were inoculated in a 24-well plate, and treated with EMS 13 h after inoculation. An aliquot (50 μL) was sampled at 5, 8, 12, and 24 h after treatment. The value at each time point was the mean of 3 wells. Vertical bars denote standard deviations from the mean. Statistically significant difference: * ( P < 0.05).

Article Snippet: Human lymphoblastoid cells (TK6) and mouse lymphoma cells (L5178Y) were obtained from the JCRB Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.

Techniques:

Journal: Dose-Response

Article Title: Collaborative Study of Thresholds for Mutagens: Hormetic Responses in Cell Proliferation Tests Using Human and Murine Lymphoid Cells

doi: 10.1177/15593258211028473

Figure Lengend Snippet: Dose-response of L5178Y cells treated with hygromycin B, aclarubicin, or colchicine. A, L5178Y cells (200,000/well/500 μL) were inoculated in a 24 well plate, treated with hygromycin B (highest dose level of 2,000 μg/mL) 12 h after inoculation, and an aliquot (50 μL) was sampled at 5, 8, 12, and 24 h after treatment. B, L5178Y cells (200,000/well/500 μL) were inoculated in a 24-well plate and treated with aclarubicin (highest dose level of 200 ng/mL) and an aliquot (50 μL) was sampled at 5, 7, 9, and 24 h after treatment. C, L5178Y cells (100,000/well/500 μL) were inoculated in a 24-well plate, treated with colchicine (highest dose level of 200 ng/mL) 13 h after inoculation, and an aliquot (50 μL) was sampled at 5, 8, 12, and 24 h after treatment. The value at each time point was the mean of 3 wells. Vertical bars denote standard deviations from the mean. Statistically significant difference: * ( P < 0.05); **( P < 0.01).

Article Snippet: Human lymphoblastoid cells (TK6) and mouse lymphoma cells (L5178Y) were obtained from the JCRB Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Japan.

Techniques: